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btg2 cdna  (Origen Biomedical)

 
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    Structured Review

    Origen Biomedical btg2 cdna
    Btg2 Cdna, supplied by Origen Biomedical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/btg2+cdna/full+length+btg2+cdna/pm34337864-81-18-21
    Average 90 stars, based on 1 article reviews
    btg2 cdna - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Exosomal microRNA-15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2/PI3K/AKT axis.
    Article Snippet: The full-length BTG2 30-untranslated region (UTR) was cloned into the SacI site of the psiCHECK-2 luciferase vector (Promega) as the Abbreviations: BTG2, B-cell translocation gene 2; F, forward; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR-15a, microRNA-15a; R, reverse; RT-qPCR, reverse transcription quantitative polymerase chain reaction. pSiBTG2 wild-type (WT) using the fragments generated by PCR, while the pSiBTG2 mutant type (MUT) vector was established as the control. .. The first five nucleotides complementary to the seed region of miR-15a were mutated by site-directed mutagenesis, and the fulllength BTG2 cDNA (OriGen Biomedical, Austin, TX) without the 30- UTR was subcloned into the eukaryotic expression vector PCMMENTRY. ..

    Expressing:

    Article Title: Exosomal microRNA-15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2/PI3K/AKT axis.
    Article Snippet: The full-length BTG2 30-untranslated region (UTR) was cloned into the SacI site of the psiCHECK-2 luciferase vector (Promega) as the Abbreviations: BTG2, B-cell translocation gene 2; F, forward; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR-15a, microRNA-15a; R, reverse; RT-qPCR, reverse transcription quantitative polymerase chain reaction. pSiBTG2 wild-type (WT) using the fragments generated by PCR, while the pSiBTG2 mutant type (MUT) vector was established as the control. .. The first five nucleotides complementary to the seed region of miR-15a were mutated by site-directed mutagenesis, and the fulllength BTG2 cDNA (OriGen Biomedical, Austin, TX) without the 30- UTR was subcloned into the eukaryotic expression vector PCMMENTRY. ..

    Plasmid Preparation:

    Article Title: Exosomal microRNA-15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2/PI3K/AKT axis.
    Article Snippet: The full-length BTG2 30-untranslated region (UTR) was cloned into the SacI site of the psiCHECK-2 luciferase vector (Promega) as the Abbreviations: BTG2, B-cell translocation gene 2; F, forward; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR-15a, microRNA-15a; R, reverse; RT-qPCR, reverse transcription quantitative polymerase chain reaction. pSiBTG2 wild-type (WT) using the fragments generated by PCR, while the pSiBTG2 mutant type (MUT) vector was established as the control. .. The first five nucleotides complementary to the seed region of miR-15a were mutated by site-directed mutagenesis, and the fulllength BTG2 cDNA (OriGen Biomedical, Austin, TX) without the 30- UTR was subcloned into the eukaryotic expression vector PCMMENTRY. ..



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    miR‐15a targets <t>BTG2</t> in ccRCC cells. (A) Targeting relation between miR‐15a and BTG2 verified by dual‐luciferase reporter gene assay (* p < 0.05; compared using two‐way ANOVA). (B) BTG2 expression in UMRC‐2 cells treated with exosomes determined by RT‐qPCR (* p < 0.05; compared using unpaired t test). (C) BTG2 expression in ccRCC tissues and adjacent normal tissues detected by RT‐qPCR (* p < 0.05; compared using paired t test). (D) Correlation analysis between miR‐15a and BTG2 in ccRCC tissues. (E) BTG2 expression in ccRCC cells and HK‐2 cells detected by RT‐qPCR (* p < 0.05; compared using one‐way ANOVA). (F) BTG2 expression quantified by RT‐qPCR following altered expression of miR‐15a (* p < 0.05, ** p < 0.01; compared using one‐way ANOVA). (G) Transfection efficiency of BTG2‐OE in cells verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (H) Effects of BTG2 on ccRCC cell proliferation determined by colony formation assay (* p < 0.05; compared using unpaired t test)
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    Image Search Results


    miR‐15a targets BTG2 in ccRCC cells. (A) Targeting relation between miR‐15a and BTG2 verified by dual‐luciferase reporter gene assay (* p < 0.05; compared using two‐way ANOVA). (B) BTG2 expression in UMRC‐2 cells treated with exosomes determined by RT‐qPCR (* p < 0.05; compared using unpaired t test). (C) BTG2 expression in ccRCC tissues and adjacent normal tissues detected by RT‐qPCR (* p < 0.05; compared using paired t test). (D) Correlation analysis between miR‐15a and BTG2 in ccRCC tissues. (E) BTG2 expression in ccRCC cells and HK‐2 cells detected by RT‐qPCR (* p < 0.05; compared using one‐way ANOVA). (F) BTG2 expression quantified by RT‐qPCR following altered expression of miR‐15a (* p < 0.05, ** p < 0.01; compared using one‐way ANOVA). (G) Transfection efficiency of BTG2‐OE in cells verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (H) Effects of BTG2 on ccRCC cell proliferation determined by colony formation assay (* p < 0.05; compared using unpaired t test)

    Journal: The Kaohsiung Journal of Medical Sciences

    Article Title: Exosomal microRNA ‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2 / PI3K / AKT axis

    doi: 10.1002/kjm2.12428

    Figure Lengend Snippet: miR‐15a targets BTG2 in ccRCC cells. (A) Targeting relation between miR‐15a and BTG2 verified by dual‐luciferase reporter gene assay (* p < 0.05; compared using two‐way ANOVA). (B) BTG2 expression in UMRC‐2 cells treated with exosomes determined by RT‐qPCR (* p < 0.05; compared using unpaired t test). (C) BTG2 expression in ccRCC tissues and adjacent normal tissues detected by RT‐qPCR (* p < 0.05; compared using paired t test). (D) Correlation analysis between miR‐15a and BTG2 in ccRCC tissues. (E) BTG2 expression in ccRCC cells and HK‐2 cells detected by RT‐qPCR (* p < 0.05; compared using one‐way ANOVA). (F) BTG2 expression quantified by RT‐qPCR following altered expression of miR‐15a (* p < 0.05, ** p < 0.01; compared using one‐way ANOVA). (G) Transfection efficiency of BTG2‐OE in cells verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (H) Effects of BTG2 on ccRCC cell proliferation determined by colony formation assay (* p < 0.05; compared using unpaired t test)

    Article Snippet: The first five nucleotides complementary to the seed region of miR‐15a were mutated by site‐directed mutagenesis, and the full‐length BTG2 cDNA (OriGen Biomedical, Austin, TX) without the 3′‐UTR was subcloned into the eukaryotic expression vector PCMM‐ENTRY.

    Techniques: Luciferase, Reporter Gene Assay, Expressing, Quantitative RT-PCR, Transfection, Colony Assay

    BTG2 can offset the effects of miR‐15a on ccRCC cells. (A) Establishment of cells overexpressing both miR‐15a and BTG2 verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (B) Cell proliferation detected by colony formation assay (* p < 0.05; compared using unpaired t test). (C) Cell migration examined by transwell assay (* p < 0.05; compared using unpaired t test). (D) Cell invasion evaluated by transwell assay (* p < 0.05; compared using unpaired t test). (E) Cell apoptosis determined by flow cytometry (* p < 0.05; compared using unpaired t test). (F) EMT activity of cells shown by detecting the florescence intensity of E‐cadherin

    Journal: The Kaohsiung Journal of Medical Sciences

    Article Title: Exosomal microRNA ‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2 / PI3K / AKT axis

    doi: 10.1002/kjm2.12428

    Figure Lengend Snippet: BTG2 can offset the effects of miR‐15a on ccRCC cells. (A) Establishment of cells overexpressing both miR‐15a and BTG2 verified by RT‐qPCR (* p < 0.05; compared using unpaired t test). (B) Cell proliferation detected by colony formation assay (* p < 0.05; compared using unpaired t test). (C) Cell migration examined by transwell assay (* p < 0.05; compared using unpaired t test). (D) Cell invasion evaluated by transwell assay (* p < 0.05; compared using unpaired t test). (E) Cell apoptosis determined by flow cytometry (* p < 0.05; compared using unpaired t test). (F) EMT activity of cells shown by detecting the florescence intensity of E‐cadherin

    Article Snippet: The first five nucleotides complementary to the seed region of miR‐15a were mutated by site‐directed mutagenesis, and the full‐length BTG2 cDNA (OriGen Biomedical, Austin, TX) without the 3′‐UTR was subcloned into the eukaryotic expression vector PCMM‐ENTRY.

    Techniques: Quantitative RT-PCR, Colony Assay, Migration, Transwell Assay, Flow Cytometry, Activity Assay

    miR‐15a‐mediated BTG2 inhibition increases activity of the PI3K/AKT pathway. Changes of PI3K/AKT pathway in exosomes‐treated cells or cells overexpressing BTG2 or/and miR‐15a detected by western blot analysis

    Journal: The Kaohsiung Journal of Medical Sciences

    Article Title: Exosomal microRNA ‐15a from ACHN cells aggravates clear cell renal cell carcinoma via the BTG2 / PI3K / AKT axis

    doi: 10.1002/kjm2.12428

    Figure Lengend Snippet: miR‐15a‐mediated BTG2 inhibition increases activity of the PI3K/AKT pathway. Changes of PI3K/AKT pathway in exosomes‐treated cells or cells overexpressing BTG2 or/and miR‐15a detected by western blot analysis

    Article Snippet: The first five nucleotides complementary to the seed region of miR‐15a were mutated by site‐directed mutagenesis, and the full‐length BTG2 cDNA (OriGen Biomedical, Austin, TX) without the 3′‐UTR was subcloned into the eukaryotic expression vector PCMM‐ENTRY.

    Techniques: Inhibition, Activity Assay, Western Blot